ABSTRACT
Objective:To determine the content of active ingredients in root bark,stems,leaves and fruits of three varieties of Sambucus williamsii (Saline-toleratedvariety,Natural Red No. 1 variety,Qingzhou No.15 variety),and identify the chemical patterns of the chemical components. Method:The content of polysaccharides,flavonoids,phenolic acids and triterpene were determined by UV spectrophotometry (UV) colorimetry. The content of anthocyanin was determined by pH differential method. Ursolic acid,oleanolic acid,quercetin,protocatechuic acid,morroniside content were determined by high performance liquid chromatography (HPLC). The root bark,stems,leaves and fruits of three varieties were analyzed by chemical pattern recognition. Result:The quality of different samples was further evaluated by F3 and F6 comprehensive scores in principal component analysis. It was found that the root bark and leaves were higher in the comprehensive ranking,and the results were basically consistent with the quantitative results. The contents of polysaccharide and morroniside in Natural Red No. 1 variety root bark were the highest,which were 161.09 mg·g-1 and 8.33 mg·g-1,respectively. Flavonoids,rutin and quercetin were the highest in the Natural Red No. 1 variety leaf,which were 71.93,4.09 and 3.57 mg·g-1,respectively. Triterpene was the highest in Natural Red No. 1 variety fruit, which was 47.84 mg·g-1. Phenolic acid and anthocyanin were the highest in Saline-tolerated variety fruits,which were 11.25 mg·g-1 and 94.32 mg·g-1,respectively. The Saline-tolerated variety stem had the highest oleanolic acid content, which was 1.41 mg·g-1. Saline-tolerated variety leaf had the highest contents of ursolic acid and gallic acid,which were 2.12 and 0.34 mg·g-1, respectively. The highest content of protocatechuic acid in Qingzhou No. 15 variety root bark, which was 0.12 mg·g-1. Conclusion:The content of Natural Red No. 1 variety is higher than that of the other two varieties,with a good quality.
ABSTRACT
Trichosanthis Fructus is a traditional Chinese herbal medicine, which has wide distribution and rich medicine source. With the in-depth study of Trichosanthis Fructus, its effective ingredients and application research has attracted much attention, and has great application prospects. Based on a summary of its chemical composition and main pharmacological effects, according to the definition of quality marker (Q-marker), the quality marker components of Trichosanthis Fructus were predicted from the aspects of the biosynthetic approach and component specificity of chemical components and their correlation with the effectiveness of Chinese medicines and the measurability of components, which provides a scientific basis for quality evaluation of Trichosanthis Fructus.